Pennsylvania State University Researcher in Hershey, Pennsylvania
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JOB DESCRIPTION AND POSITION REQUIREMENTS:
A Researcher junior faculty non-tenure track position is immediately available to study the impact of arrhythmia and heart failure in congenital heart disease. Our laboratory uses multidisciplinary approaches to study the structural and electrical components of cardiovascular function in adults surviving congenital heart disease. Our research spans from human to animal, employing novel imaging and electrophysiologic techniques, to name a few. The successful candidate will employ small animal echocardiography skills, murine surgical skills to implant radiotelemetry devices and perform transaortic constriction, monitoring of electrocardiographic data and catecholaminergic stress testing, as well as basic laboratory techniques to perform immunoblotting assays, fluorescence imaging, fluorescence-based studies employing a robotics microplate reader, quantitative PCR, gel electrophoresis and cell culture techniques. The successful candidate must demonstrate technical and theoretical knowledge of the aforementioned assays. As a consequence, the candidate will be expected to analyze the data collected and present the results in formal presentations, manuscripts and grant applications.
% of time
Perform small animal cardiac structure and functional analysis. This includes independently performing small animal echocardiography, real-time measurements of function using 2D, M-mode, & Doppler technology, as well as analysis of data. Perform radiotelemeter implantation, resting electrocardiographic studies with measurement of appropriate intervals & segments along with analysis of this data, and finally, catecholaminergic stress testing with associated interval/segment measurement & analysis.
Prepare cDNA clones for transfection, prepare dissection and dissociation solutions, process DNA and protein samples for PCR and Western blotting assays. Maintain cell line cultures for transfection. Transfect cell lines, perform pharmacological assays of transfected cell lines with the microplate reader, perform Western blotting assays and quantitative RT-PCR assays.
Prepare human iPSC derived cardiomyocytes and perform contraction analyses at rest and in response to medication studies.
Independently monitor and carry out oversight of animal lines including transgenic mice colony maintenance, employing gel electrophoresis and PCR techniques, preparing solutions and other routine lab duties. Analyze and plot data obtained from the assays performed.
Provide training of other technicians to ensure that duties above are properly and aptly executed.
Position Specific Competencies
Knowledge of lab practices and procedures in animal handling, dissection, Western blotting, PCR, fluorescence imaging, sub-cloning, and transfection (employing lipofection, nuclear microinjection or electroporation).
Accountability & Self-Management
Work independently, with minimal supervision by PI. Perform routine duties using established procedures with coordination and effective communication with the PI.
Teamwork & Leadership
Independently develop and implement laboratory policies, update standard operating procedures that should ensure safe practices for all laboratory members.
Communicate effectively with PI and other laboratory members as the projects are executed. Excellent verbal and written communication required for manuscript and grant preparation.
Innovation & Problem Solving
Will contribute to the optimization of experimental procedures that require novel approaches.
The Pennsylvania State University is committed to and accountable for advancing diversity, equity, and inclusion in all of its forms. We embrace individual uniqueness, foster a culture of inclusive excellence that supports both broad and specific diversity initiatives, leverage the educational and institutional benefits of diversity, and engage all individuals to help them thrive. We value inclusive excellence as a core strength and an essential element of our public service mission.
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Employment with the University will require successful completion of background check(s) in accordance with University policies.
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